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The 1x TAE solution is 40mM Tris, 20mM Acetate and 1mM EDTA and typically has a pH around 8.6 (do not adjust). Add 59.955 g of Acetic Acid to the solution. Add 18.61 g of Disodium EDTA to the solution.

Journal of Medical Genetics 27 (9): 569-570. Prepare 800 mL of dH2O in a suitable container. "Repeated freezing and thawing of peripheral blood and DNA in suspension: effects on DNA yield and integrity". Repeated freeze-thaw cycles should be avoided. Genomic and plasmid DNA can be stored in TE Buffer at 4✬ (39.2✯) for short-term use, or -20✬ (-4✯) to -80✬ (-112✯) for long-term storage. The respective DNA and RNA nucleases are supposed to be less active at these pH values, but pH 8.0 can safely be used for storage of both DNA and RNA.ĮDTA further inactivates nucleases, by binding to metal cations required by these enzymes. To make a 100 ml solution of T 10E 1 Buffer, 1 ml of 1 M Tris-HCl (pH 8.0) and 0.2 ml EDTA (0.5 M) and made up with double distilled water up to 100ml.īased on nuclease studies from the 1980's, the pH is usually adjusted to 7.5 for RNA and 8.0 for DNA. TE buffer is also called as T 10E 1 Buffer, and read as "T ten E one buffer". RecipeĪ typical recipe for making 1X TE buffer is:

The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg 2+. TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA.